Table 1. A review of plasma preparation protocols in extracellular miRNA-disease association studies.

Study	Disease	AC	Freeze before platelet removal	Whole blood cent. speed	Time (min)	2nd cent. speed	Time (min)	Yr.	PMID	Journal
Wong et al.	HFpEF	EDTA	Yes	3,488x g	10	No		2015	25619197	Eur J Heart Fail	B
Aherne et al.	Colorectal cancer	EDTA	Yes	1,000x g	10	No		2015	25924769	BMC Cancer	C
Melman et al.	Response to CRT	?	?	?	?	?	?	2015	25995320	Circulation	C
Ameling et al.	Associations with age, BMI, sex	EDTA	?	?	?	?	?	2015	26462558	BMC Med Genomics	C
Jaguszewski et al.	Takotsubo cardiomyopathy	EDTA	Yes	1,300x g	15	No		2014	24046434	Eur Heart J.	C
Xiao et al.	Acute graft-versus-host disease	?	No	2,000 RPM	10	12,000 RPM	3	2014	24041574	Blood	A
Garcia et al.	Regression of LVH	EDTA	Yes	1,389x g	30	No		2014	23948643	JAHA	C
Roncarati et al.	Hypertrophic cardiomyopathy	EDTA	Yes	1,100x g	20	No		2014	24161319	JACC	C
Zanutto et al.	Colorectal cancer	EDTA	Yes	3,000x g	10	1,000x g	5	2014	24423916	Br J Cancer	C
Huang et al.	Myocardial infarc	EDTA	No	1,600x g	10	16,000x g	10	2014	24627568	Circ. CV Genet	A
Morely-Smith et al.	Response to LVAD	?	?	?	?	?	?	2014	24961598	Eur J Heart Fail	C
Ward et al.	Hepatotoxicity or ischemic hepatitis	EDTA	Yes	1,500x g	20	No		2014	25092309	PNAS	C
Pangxiang et al.	Intracranial aneurysm	EDTA	No	1,600x g	10	13,000x g	10	2014	25249297	JAHA	A
Jansen et al.	Cardiovascular events	Citrate	No	1,500x g	15	13,000x g	2	2014	25349183	JAHA	A
de Boer et al.	Aspirin effect on miR-126	Citrate	Yes	2,700x g	15	No		2013	23386708	Eur Heart J.	B
Dawson et al.	Atrial fibrillation	EDTA	Yes	4,000 RPM	20	No		2013	23459615	Circulation	B
Williams et al.	Comprehensive sequencing	EDTA	No	500x g	5	16,060x g	5	2013	23440203	PNAS	A
Ellis et al.	Heart failure	EDTA	Yes	?	?	No		2013	23696613	Eur J Heart Fail	C
Ferrajoli et al.	B-cell lymphocytosis and B chronic lymphocytic leukemia	Citrate	?	?	?	?	?	2013	23821659	Blood	C
Lee et al.	Recurrent papillary thyroid cancer	EDTA	Yes	800x g	15	No		2013	24301304	Cancer	C
Zampetaki et al.	Myocardial Infarction	?	?	?	?	?	?	2012	22813605	JACC	C
Kin et al.	AAA	?	?	?	?	?	?	2012	23316282	JAHA	C
Moussat et al.	B-cell chronic lymphocytic leukemia	EDTA	No	1,000x g	10	10,000x g	20	2011	21460253	PNAS	A
Boeri et al.	Lung cancer	EDTA	No	1,258x g	?	1,258x g	?	2011	21300873	PNAS	A
Zuo et al.	Myelodysplastic syndrome	?	?	?	?	?	?	2011	21602527	Blood	C
Li et al.	Hypertension	EDTA	?	1,000x g	10	No		2011	21690488	Circulation	C
Komatsu et al.	Esophageal cancer	?	No	1,500 RPM	30	3,000 RPM	5 min	2011	21673684	Br J Cancer	C
Morimura et al.	Pancreatic cancer	Heparin	No	1,500 RMP	30	3,000 RPM	5 min	2011	22045190	Br J Cancer	C
De Rosa et al.	Transcoronary grandient	?	?	?	?	?	?	2011	21969012	Circulation	C
Zile et al.	Left ventricular remodeling	?	?	?	?	?	?	2011	21956146	Circ. CV Genet	C
Wang et al.	Acute myocardial infarction	EDTA	No	820x g	10	16,000x g	10	2010	20159880	Eur Heart J.	A
Tijsen et al.	Heart failure	Citrate	Yes	1,550x g	10	No		2010	20185794	Circ Res	B
Ho et al.	Pancreatic cancer	?	Yes	3000 RPM	10	13,000x g	10	2010	20360935	Transl Oncol.	C
Tsujiura et al.	Gastric cancer	?	?	?	?	?	?	2010	20234369	Br J Cancer	C
Zampetaki et al.	Tyoe 2 diabetes	?	Yes	?	?	?	?	2010	20651284	Circ Res	C
Fichlscherer et al.	Coronary artery disease	EDTA	?	?	?	?	?	2010	20595655	Circ Res	C
D’Alessandra et al.	Myocardial infarction	EDTA	No	1,500x g	15	14,000x g	15	2010	20534597	Eur Heart J.	A
Maarten et al.	Myocardial damage	Citrate EDTA	Yes	?	?	?	?	2010	20921333	Circ CV Genet.	C
Wang et al.	Drug induced liver injury	?	?	?	?	?	?	2009	19246379	PNAS	C
Tanaka et al.	Acute leukemia	?	no	1,600x g	15	No		2009	19440243	PLoS One	C
Mitchell PS et al.	Prostate cancer	EDTA	yes	1,200x g	10	No		2008	18663219	PNAS	C
Ng et al.	Colorectal cancer	EDTA	no	1,600x g	10	16,0000x g	10	2008	19201770	Gut	A

AC = anticoagulant; A = likely removed platelets; B = unclear whether platelets were removed; C = unlikely platelets were removed or not enough detail in methods to determine.