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. 2016 Aug;11(8):1260–1266. doi: 10.4103/1673-5374.189190

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Time course of caspase-3 expression in traumatic penumbra at 6, 12, 24, and 72 hours (h) after traumatic brain injury (TBI).

(A) Western blot. Optical density of caspase-3 bands was normalized to that of β-actin. (B) Representative photomicrographs showing elevated immunoreactivity of caspase-3 in traumatic penumbra after TBI. Arrows: Caspase-3-positive cells. Bar: 50 μm. (C) Quantification of caspase-3 immunonreactivity (average optical density, AOD) in traumatic penumbra. Data are expressed as the mean ± SD, n = 6 rats per group.*P < 0.05, vs. sham-operated (sham) group (one-way analysis of variance with Tukey's test).