Table 4.

Trend analysis by Gleason score

	Gleason 5		Gleason 6		Gleason 7		Gleason 8		Gleason 9		p Value (difference)*	p Value (trend)†
No. Cases	6		142		52		9		8
freePSA (ng/ml):
Mean (SD)	0.7	(0.2)	0.7	(0.5)	0.9	(0.7)	3.1	(3.8)	1.7	(2.2)	0.131	<0.001
Median (Q1, Q3)	0.7	(0.6, 0.9)	0.6	(0.5, 0.9)	0.8	(0.5, 1.1)	0.8	(0.6, 6.1)	0.9	(0.5, 1.9)
[−2]proPSA (pg/ml):
Mean (SD)	12.9	(2.9)	12.8	(7.8)	18.2	(16.2)	93.7	(145.5)	39.6	(49.1)	0.002	<0.001
Median (Q1, Q3)	13.2	(10.1, 15.5)	11.1	(7.5, 16.7)	14.9	(10.1, 20.3)	19	(11, 130.3)	24.8	(12.4, 41.4)
phi:
Mean (SD)	33.9	(9.4)	33.5	(16.4)	52.2	(49)	113.8	(149.1)	89.8	(71.4)	<0.001	<0.001
Median (Q1, Q3)	31.5	(27.9, 33.6)	29.5	(22.2, 40)	37.5	(27.9, 61.6)	50.3	(39.1, 106.4)	52.3	(38.8, 146.8)
%freePSA:
Mean (SD)	22	(7.3)	23.3	(12.1)	18.8	(11)	17.5	(5.6)	13.7	(9.5)	0.006	<0.001
Median (Q1, Q3)	21.8	(16, 28.2)	20	(14.8, 29.1)	16	(9.8, 24.6)	16.2	(15.5, 17.4)	11.1	(7.7, 16.1)
%[−2]proPSA:
Mean (SD)	1.9	(0.7)	1.8	(0.7)	2.1	(0.9)	2.8	(1.9)	2.6	(1.3)	0.113	0.002
Median (Q1, Q3)	1.6	(1.5, 2)	1.8	(1.4, 2.1)	1.9	(1.5, 2.5)	2.1	(2, 2.7)	2.2	(1.6, 3.4)
DKK1 (ng/ml):
Mean (SD)	1.07	(0.49)	1.18	(0.6)	1.17	(0.98)	1.24	(0.45)	1.62	(1.24)	0.57	0.544
Median (Q1, Q3)	1.05 (0.95, 1.13)		1.16 (0.76, 1.44)		0.96 (0.67, 1.35)		1.11	(0.89, 1.58)	1.09	(0.83, 2.18)
NEM (ng/ml):
Mean (SD)	0.21	(0.23)	0.95	(4.08)	1.33	(3.3)	5.31	(10.97)	0.22	(0.2)	0.486	0.372
Median (Q1, Q3)	0.08 (0.07, 0.37)		0.18 (0.06, 0.64)		0.15 (0.05, 0.61)		0.57	(0.12, 1.15)	0.13	(0.07, 0.39)

^*Test of difference among Gleason score categories using the Kruskal-Wallis test.

^†Test of linear trend of log transformed markers across Gleason score categories.