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. 2016 Aug 25;107(9):1198–1205. doi: 10.1111/cas.12985

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© 2016 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Antibody‐dependent cellular cytotoxicity (ADCC) activity of rat anti–human podoplanin antibody NZ‐1 against NCI‐H290/PDPN in vitro. (a) Expression of podoplanin was detected by FACS analysis. (b) ADCC activity of NZ‐1 using rat splenocytes against NCI‐H290/PDPN or NCI‐H290/Mock was evaluated by 6‐h ⁵¹Cr release assay in the presence of antibody (1 μg/mL; effector/target [E/T] ratio 100). (c) E/T ratio‐dependent and antibody dose‐dependent effects of ADCC against NCI‐H290/PDPN mediated by NZ‐1 with rat splenocytes are shown by ⁵¹Cr release assay. (d) Rat natural killer (NK) (CD161a⁺) cells were isolated from rat splenocytes. ADCC activity of NZ‐1 (1 μg/mL) mediated by NK (CD161a⁺) cells was evaluated by 6‐h ⁵¹Cr release assay at an E/T ratio of 10. **P < 0.01 versus control (values represent mean ± SE). rIgG, rat IgG.