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. 2016 Aug 25;107(9):1198–1205. doi: 10.1111/cas.12985

Figure 4.

Figure 4

Antitumor activity of rat–human chimeric anti‐human podoplanin antibody NZ‐8 in an NCI‐H290/PDPN malignant pleural mesothelioma orthotopic xenograft model. (a) Antibody‐dependent cellular cytotoxic activity against NCI‐H290/PDPN was determined with 6‐h 51Cr release assay (effector/target ratio 100) in the presence of human IgG (hIgG; 1 μg/mL), NZ‐1 (1 μg/mL), or NZ‐8 (1 μg/mL), with human peripheral blood mononuclear cells (MNC). (b) NCI‐H290/PDPN (1.0 × 106 cells) was inoculated into the thoracic cavity on day 0 (n = 5). NZ‐8 (100 μg) or hIgG (100 μg) injection (i.p.) also began on day 0, and continued twice a week for 2 weeks. Human natural killer (NK) (CD56+) cells (1.0 × 106 cells) or control normal saline were injected into the thoracic cavity from day 3, and continued weekly for 2 weeks. Mice were killed 3 weeks after tumor cell inoculation. *P < 0.05, **P < 0.01 (values represent mean ± SE).