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. Author manuscript; available in PMC: 2016 Sep 13.
Published in final edited form as: Mol Cancer Res. 2016 Feb 5;14(4):363–373. doi: 10.1158/1541-7786.MCR-15-0399

Figure 3. Double-strand break repair activity correlates with miR-155 expression.

Figure 3

(A) AV16 cells constitutively expressing NTC (black) or 155 (grey) and (B) MEFs either WT (black) or KO (grey) for the BIC locus containing the miR-155 gene were analyzed for double-strand breaks using the neutral Comet assay. Error bars were calculated as standard error of the mean. P-value was calculated by unpaired t-test (n=3). (C) Schematic representation of the luciferase reactivation assay measuring NHEJ or HR activity. NHEJ or HR luciferase reactivation was compared to a positive control to determine percent reactivation. A Renilla luciferase plasmid was used to control for transfection efficiency. (D) AV16 NTC and 155 cells were analyzed for NHEJ and (E) HR activity using luciferase reactivation assays. MEFs WT or KO for miR-155 were analyzed for (F) NHEJ and (G) HR activity using the luciferase reactivation assays.