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. 2016 Sep 13;6:32992. doi: 10.1038/srep32992

Figure 5. Crizotinib, BKM120 and GDC-0980 alone or in combination inhibit the anchorage independent growth of mesothelioma cells in a soft agar assay.

Figure 5

H2596 cells were seeded in 0.4% Agarose and treated with 1 μM crizotinib, 2 μM BKM120, 0.25 μM GDC-0980 alone and in combination for a period of 30 days. Images of colonies were taken and colonies were counted using Image J. (A) Results are expressed as number of colonies per field of view ± SEM. Results were analyzed by ANOVA with Tukey post-test. (NS p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001) (BKM 120 Vs BKM120/crizotinib p < 0.001 GDC-0980 Vs GDC-0980/crizotinib p < 0.05). (B) Representative images of colonies. (C) Colony size in pixels for different groups ± SEM. (BKM120 Vs BKM120/crizotinib p < 0.01 GDC-0980 Vs GDC-0980/crizotinib p < 0.05) (D). Images of colonies were taken using a Zeiss Axiovert 200 m inverted optical microscope. Representative images of single colonies are shown at 10x magnification. Scale bar = 20 μm.