Table 2.

Comparison of T‐DNA copy number estimated using ddPCR or qPCR estimations of PCR template concentration of six different primersets targeting T‐DNA regions and either actin or α‐tubulin as a reference gene. Analysis was performed on digested DNA of VPZ‐46 T_1.5 plant carrying a T‐DNA with three genes (AtVDE, AtPsbs and AtZEP). (± se; n = 4 for qPCR; n = 2 for ddPCR).

	qPCR		ddPCR
	T‐DNA copy number		T‐DNA copy number
Primer set name	actin	α‐tubulin	actin	α‐tubulin
AtPsbS_3	1.49 ± 0.11	2.85 ± 0.25	2.01 ± 0.05	1.82 ± 0.03
AtPsbS_4	1.86 ± 0.49	2.29 ± 0.06	2.19 ± 0.06	1.98 ± 0.03
AtVDE_1	2.08 ± 0.26	3.19 ± 0.13	2.26 ± 0.12	2.05 ± 0.06
AtVDE_4	3.51 ± 0.20	5.38 ± 0.10	2.06 ± 0.05	1.87 ± 0.02
AtZEP_1	2.01 ± 0.18	3.08 ± 0.09	2.16 ± 0.08	1.96 ± 0.04
AtZEP_4	1.92 ± 0.09	2.94 ± 0.05	2.16 ± 0.11	1.96 ± 0.06
Variance per method	1.10		0.02