Table 4.
Results of screening for copy number to identify homozygous plants in T1 segregating progenies with the use of ddPCR. Seedlings of T1 lines were sprayed with glufosinate ammonium solution before single plants were transferred to pots and grown for tissue collection for ddPCR analyses. Bold numbers indicate homozygous plants identified by a duplication of the copy number in the corresponding T0 plant (± se; n = 2).
Generation | Transformation event | |||
---|---|---|---|---|
PsbS‐43 | PsbS‐46 | VPZ‐29 | VPZ‐34 | |
T0 | 1.09 ± 0.13 | 0.98 ± 0.18 | 0.97 ± 0.09 | 0.97 ± 0.14 |
T1 | 2.10 ± 0.22 | 2.04 ± 0.16 | 1.96 ± 0.16 | 0.94 ± 0.21 |
2.13 ± 0.20 | 1.08 ± 0.11 | 0.93 ± 0.15 | 1.93 ± 0.06 | |
2.05 ± 0.04 | 1.01 ± 0.28 | 0.98 ± 0.16 | 2.08 ± 0.06 | |
1.12 ± 0.25 | 2.08 ± 0.10 | 1.04 ± 0.20 | 0.98 ± 0.09 | |
2.02 ± 0.16 | 2.09 ± 0.08 | 1.03 ± 0.27 | 1.80 ± 0.21 | |
2.04 ± 0.05 | 2.04 ± 0.05 | 0.95 ± 0.08 | 1.87 ± 0.11 | |
2.26 ± 0.04 | 2.10 ± 0.08 | 1.03 ± 0.10 | 1.80 ± 0.31 | |
2.10 ± 0.28 | 1.08 ± 0.26 | 1.02 ± 0.03 | 2.02 ± 0.20 |