Table 5.
T‐DNA copy (± se; n = 2) estimated by digital droplet PCR (ddPCR) and estimated number of T‐DNA loci based on segregation analyses (Chi2‐test for 1:3 segregation, α = 0.1). Segregation was scored based on either resistance to glufosinate ammonium (GA) in 17‐day‐old seedlings or level of non‐photochemical quenching (NPQ) in 7‐day‐old seedlings. NPQ values were induced by 10 min exposure to PFD of 1000 μmol quanta m‐2 s‐1.
| ddPCR | Segregation of resistance to GA leaf paint in T1 generation | Segregation based on NPQ in Fig. 2b | |||||
|---|---|---|---|---|---|---|---|
| ID of T0 plant | No. of T‐DNA copy | No. of susceptible plants (%) | No. of resistant plants (%) | No. of T‐DNA loci in line | No. of plants with WT NPQ (%) | No. of plants with NPQ higher than WT (%) | No. of T‐DNA loci in line |
| PsbS‐23 | 0.99 ± 0.17 | 6 (27) | 16 (73) | 1 | 2 (11) | 16 (89) | 1 |
| PsbS‐25 | 0.98 ± 0.06 | 8 (33) | 16 (67) | 1 | 2 (12) | 15 (88) | 1 |
| PsbS‐27 | 0.97 ± 0.04 | 5 (23) | 17 (77) | 1 | 7 (41) | 10 (59) | 1 |
| PsbS‐28 | 1.04 ± 0.10 | 5 (21) | 19 (79) | 1 | 3 (17) | 15 (83) | 1 |
| PsbS‐34 | 0.87 ± 0.22 | 4 (17) | 20 (83) | 1 | 1 (5) | 18 (95) | >1 |
| PsbS‐43 | 1.09 ± 0.13 | 5 (21) | 19 (79) | 1 | 4 (22) | 14 (78) | 1 |
| PsbS‐47 | 0.98 ± 0.18 | 3 (12.5) | 21 (87.5) | 1 | 4 (21) | 15 (79) | 1 |
| PsbS‐49 | 1.99 ± 0.21 | 0 (0) | 23 (100) | >1 | 1 (6) | 17 (94) | >1 |
| PsbS‐50 | 1.97 ± 0.10 | 3 (12.5) | 21 (87.5) | 1 | 4 (22) | 14 (78) | 1 |
| PsbS‐2 | 3.94 ± 0.21 | 0 (0) | 24 (100) | >1 | 0 (0) | 15 (100) | >1 |
| 1 (1), a | 71 (99)a | >1a | |||||
| PsbS‐32 | 3.89 ± 0.05 | 6 (26) | 17 (74) | 1 | 4 (29) | 10 (71) | 1 |
| VPZ‐14 | 1.03 ± 0.38 | 5 (21) | 19 (79) | 1 | na | na | na |
| 19 (26)a | 53 (74)a | 1a | |||||
| VPZ‐29 | 0.97 ± 0.09 | 6 (26) | 17 (74) | 1 | na | na | na |
| VPZ‐31 | 1.07 ± 0.09 | 5 (21) | 19 (79) | 1 | na | na | na |
| VPZ‐34 | 0.97 ± 0.14 | 8 (33) | 16 (67) | 1 | na | na | na |
| VPZ‐38 | 1.02 ± 0.05 | 2 (9) | 21 (91) | >1 | na | na | na |
| VPZ‐54 | 0.97 ± 0.07 | 9 (37.5) | 15 (62.5) | 1 | na | na | na |
| VPZ‐13 | 2.12 ± 0.08 | 5 (23) | 17 (77) | 1 | na | na | na |
| 20 (28)a | 51 (72)a | 1a | |||||
| VPZ‐23 | 1.83 ± 0.11 | 4 (17) | 20 (83) | 1 | na | na | na |
| 18 (25)a | 54 (75)a | 1a | |||||
| VPZ‐28 | 1.91 ± 0.16 | 2 (8) | 22 (92) | >1 | na | na | na |
| VPZ‐35 | 2.94 ± 0.13 | 1 (4) | 23 (96) | >1 | na | na | na |
| VPZ‐50 | 2.86 ± 0.05 | 1 (4) | 23 (96) | >1 | na | na | na |
| VPZ‐52 | 2.88 ± 0.17 | 2 (8) | 22 (92) | >1 | na | na | na |
| VPZ‐51 | 4.83 ± 0.14 | 4 (17) | 20 (83) | 1 | na | na | na |
| VPZ‐33 | 11.96 ± 0.13 | 15 (62.5) | 9 (37.5) | nab | na | na | na |
| VPZ‐36 | 12.09 ± 0.08 | 0 (0) | 24 (100) | >1 | na | na | na |
a
Data collected of resistance to leaf‐paint of GA solution in 72 field‐grown plants.
b
Not estimated because of non‐Mendelian segregation. na – not applicable.