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. 2016 Apr 1;54(5):286–296. doi: 10.1002/dvg.22937

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© 2016 The Authors. Genesis Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Generation of tissue‐specific centrosome indicator. A: Design of targeting vector for ROSA locus. Human Centrin1 cDNA fused with EGFP coding sequence at N‐terminus was placed after a floxed Neomycin resistant gene and triplicated SV40 poly A cassette. B: Southern blot for ES clones. C: PCR with mouse tail DNA after germ‐line transmission. D: Southern blot with mouse tail DNA, indicating successful germ‐line transmission. E: ED9.5 embryo of ROSA‐Neo‐EGFP‐Cetn1 mouse line after crossing with Nkx2.5‐Cre, indicating lineage specific expression of EGFP‐Centrin1. (a1, b1, c1): bright field, (a2, b2, c2): EGFP‐Centrin1. Scale bar: 1 mm.