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. 2016 Apr 1;54(5):286–296. doi: 10.1002/dvg.22937

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© 2016 The Authors. Genesis Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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EGFP‐Centrin1 in adult mouse. A, B: Intestine tissue sections of ROSA‐EGFP‐Cetn1;Rosa‐mTmG were immunostained with the antibody to pH3 or E‐cadherin. C: Skin tissue sections of ROSA‐EGFP‐Cetn1;Rosa‐mTmG were immunostained with antibody to E‐cadherin. D, E, F, G, H: Kidney (D, E) and testis (F, G, H) tissue sections of ROSA‐EGFP‐Cetn1 were immunostained with antibody to acetylated‐Tubulin. (G, H): Magnified pictures of square areas of (F). H: Extensive green signal was observed in extracellular matrices, presumably caused by auto‐fluorescence. I: Isolated sperm of ROSA‐EGFP‐Cetn1 was immunostained with antibody to acetylated Tubulin. Note EGFP‐Centrin1 signal is located at the base of flagella. Scale bars: 20 µm (A, B, C, D, F), 5 µm (G, H, I).