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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Diabetes Obes Metab. 2016 Sep;18(Suppl 1):33–40. doi: 10.1111/dom.12720

Figure 2. Adult spheres and further endocrine differentiation.

Figure 2

Spheres forming from mouse and human adenocarcinoma (a) [57] [34], adult murine ducts (b) [39] (c) [38], or human pancreatic ducts (d) [40]), islets (e) [47, 48] or aldefluor-labelled exocrine/terminal duct cells (f) [51]. They are expanded in semisolid matrixes (Matrigel) (a-d) or in suspension on non-adhesive plates (e, f) and generate dense (c) or hollow (a-f) spheres in presence of the soluble factors indicated. Different approaches have been used to generate endocrine cells from these cultures, as indicated. Modified from [74].