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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Nature. 2016 May 18;533(7604):493–498. doi: 10.1038/nature18268

Extended Figure 5. Inhibition of gap junction activity prevents brain metastatic outgrowth.

Extended Figure 5

a, Bioluminescent imaging (BLI) quantification of brain metastatic lesions formed by control (Ctrl), Cx43- or PCDH7-depleted H2030-BrM3 cells. (Data are from 2 independent experiments with 9 mice total per group). b, Representative images of GFP+ brain metastatic lesions formed by control, Cx43- or PCDH7-depleted MDA231-BrM2 cells. Brain sections or brain metastatic lesions are delineated by dotted white line or dotted red line, respectively. Scale bar, 1000 μm. c, BLI (images) and quantification (bar graph) of lung metastatic lesions formed by MDA231-BrM2 cells. Values are mean ± S.E.M. (Data are from 2 independent experiments with 5 mice total in each group). d,e, Gap junction-mediated brain metastasis requires channel function of Cx43. Wild type (WT) or T154A mutant (Mut) Cx43 was re-expressed in Cx43 depleted MDA231-BrM2 cells (CX43 sh2). Cx43 expression was detected by western blotting (d) and brain metastasis formed by these cells was quantified by BLI (e). (Data are from 2 independent experiments with 10 mice total per group)