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. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Nature. 2016 May 18;533(7604):493–498. doi: 10.1038/nature18268

Figure 2. Cx43/PCDH7 carcinoma-astrocyte gap junctions mediate brain metastasis.

Figure 2

a, Histograms (top) and quantification (bottom) of dye transfer from astrocytes to control and Cx43-depleted or PCDH7-depleted brain metastatic cells. Values are mean ± S.E.M. (Data are from n=3 biological replicates over 3 independent experiments). b-d, BLI (c) and quantification (b, d) of brain metastatic lesions formed by control, Cx43-depleted, or PCDH7-depleted brain metastatic cells in the MDA231 xenograft model or 393N1 syngeneic models of brain metastasis. Data are from n=3 independent experiments, n=8-10 mice per group. All source data from mouse experiments are in Supplementary Information. e,f, Wild type (WT) or T154A mutant (Mut) Cx43 was re-expressed in Cx43-depleted MDA231-BrM2 cells (Cx43 sh2). The cells were subjected to astrocyte dye transfer analysis by flow cytometry (e, 3 independent experiments), or to brain metastasis assays and BLI quantification (f, n=2 independent experiments, 9 mice per group). g, Schematic summary of Cx43- and PCDH7-mediated interactions between cancer cells and astrocytes in brain metastasis.