Skip to main content
. Author manuscript; available in PMC: 2016 Nov 18.
Published in final edited form as: Nature. 2016 May 18;533(7604):493–498. doi: 10.1038/nature18268

Extended Figure 3. PCDH7 facilitates gap junction communication.

Extended Figure 3

a,b, Histograms and quantification of dye transfer from astrocytes to control and Cx43-depleted or PCDH7-depleted Kras/p53-393N1 cells (a), and from astrocytes to control or Cx43-depleted MDA231-BrM2 cells, in comparison to Carbenoxolone (50 uM) treatment (b). c,d, PCDH7 in astrocytes facilitate gap junctions. PCDH7 immunoblotting of control or PCDH7-depleted astrocytes (c). Quantification of dye transfer from MDA231-BrM2 cells to PCDH7-depleted astrocytes (d). e, Quantification of dye transfer from human brain microvascular endothelial cells (HBMEC) to control, Cx43- or PCDH7-depleted MDA231-BrM2 cells. f, Dye transfer from MDA231-BrM2 cells to a mixed population of astrocytes and HBMEC. g, Quantification of dye transfer from control or Cx43-depleted MDA231-BrM2 cells to human microglia. For dye transfer assays, values are mean ± S.E.M. (≥ 2 independent experiments in triplicate).