Fig. 5.

The number of dopaminergic neurons in the substantia nigra pars compacta (SNpc) of 2-year-old wild-type and various synuclein knockout mice. (A) The bar chart shows means ± standard error of the mean of stereologically counted TH-positive neurons per hemisphere. Asterisks denote statistically significant difference between knockout and wild-type groups (^∗p < 0.05, ^∗∗p < 0.01, Kruskal–Wallis analysis of variance with post hoc Mann–Whitney test). The number of samples processed for each group is shown at the bottom of the corresponding bar. (B) Representative images of anti-TH immunostained sections through the midbrain of the wild-type and synuclein deficient mice at the level between Bregma −3.0 to −3.2, where the border between SNpc and ventral tegmental area is well defined. Insets show SNpc dopaminergic neurons at higher magnification.