Skip to main content
. 2016 Aug 3;49(4):1394–1406. doi: 10.3892/ijo.2016.3646

Figure 1.

Figure 1

SPARC upregulation combined with radiation reduces VEGF-mediated angiogenesis in vivo. Expression of VEGF-A and SPARC was determined on human tissue array using the HRP substrate DAB. (A) Tissue arrays were processed for immunohistochemistry and expression of VEGF-A and SPARC are shown. Angiogenic potential of SK-N-BE(2) and NB1691 cell lines was determined using the dorsal skin fold assay. (B) Pre-existing vasculature (PV) and tumor-induced vasculature (TV) are indicated. (C) TV was quantified as density of newly formed vessels and represented as arbitrary pixel units (control vs. SPARC and control vs. SPARC + radiation, *P<0.05, **P<0.001). To determine the most prominent pro-angiogenic molecule expressed in SK-N-BE(2) and NB1691 cell lines, RT-PCR analysis of known pro-angiogenic molecule was done. Expression levels of pro-angiogenic mRNA levels were quantified and graphically represented (D).