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. 2016 Sep 13;11(9):e0162875. doi: 10.1371/journal.pone.0162875

Fig 4. Lack of antinociceptive tolerance of YHS (200 mg/kg) in the tail flick assay (n = 7–8).

Fig 4

Two way ANOVA revealed a significant drug effect (F2,19 = 68.24, P < 0.0001), time effect (F4,76 = 19.16, P < 0.0001) and drug x time interaction (F8,76 = 7.509, P < 0.0001) followed by Bonferroni post hoc test: drug vs vehicle, *P < 0.05, **P < 0.01, *** P < 0.001. One way ANOVA revealed a significant time effect in morphine treated group (F3,24 = 5.520, P = 0.005) but not in YHS treated group (F3,28 = 0.6032, P = 0.6184) followed by Dunnett’s post hoc tests: Day 3,5,or 7 vs Day 1, ## P < 0.01, ### P < 0.001, N.S., not significant.