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. 2016 Sep 13;11(9):e0162544. doi: 10.1371/journal.pone.0162544

Fig 2. Patterns of rDNA organization, 45S rDNA loci number, and nucleolar activity in species of Marchantiophyta, Bryophyta, and Anthocerotophyta (liverworts, mosses and hornworts, respectively).

Fig 2

A-E, FISH of rRNA genes using the 45S rDNA probe. F Ag-NOR staining. G-I, Sequential Ag-NOR and FISH. A, Thuidium delicatulum. B, Pellia epiphylla and C, Neckera crispa showing a single site signal. D, Pellia endiviifolia and E, Campylopus introflexus, showing two sites. F, Ag-NOR staining in interphase nuclei of Pellia epiphylla showing one large nucleolus. G-H, Sequential Ag-NOR (G) and FISH (H) in Atrichum undulatum. The Ag-staining in several interphase nuclei shows one nucleolus (top right), two homomorphic (left bottom), and two heteromorphic nucleoli (middle). I, merged G and H images. Scale bars: 10 μm.