(a) Real-time RT-PCR analysis following siRNA knockdown of ILF3 in HeLa cells. The data points are the average of duplicate samples. (b) Real-time RT-PCR analysis of SMN transcripts including exon 7 after treating HeLa cells with increasing concentrations of ILF3 siRNA for 48 h and then another 24 h with a fixed concentration of ASO. The data points are the average of duplicate samples. Black solid line, ILF3 knockdown and no ASO; blue solid line, no ILF3 knockdown and 2′-F ASO; blue dashed line, ILF3 knockdown and 2′-F ASO; red solid line, no ILF3 knockdown and 2′-MOE ASO; red dashed line, ILF3 knockdown and 2′-MOE ASO. (c) Same as in b, except that the real-time RT-PCR analysis is for SMN transcripts excluding exon 7.