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. Author manuscript; available in PMC: 2016 Sep 13.
Published in final edited form as: Nat Chem Biol. 2012 Apr 15;8(6):555–561. doi: 10.1038/nchembio.939

Figure 5. Effect of 2′-F mixmer ASOs on exon skipping.

Figure 5

(a) Structure of the mixmer ASOs. F is 2′-F. X is either DNA, RNA, 2′-OMe, 2′-MOE or cEt. For each ASO, every X has the same chemistry. (b) Real-time RT-PCR analysis of SMN2 transcripts including exon 7 following ASO treatment of fibroblasts from patients with SMA. The concentration of transfected ASO is shown above the graph. The data points are the average of duplicate samples. (c) Same as in b, except that the real-time RT-PCR analysis is for SMN2 transcripts excluding exon 7.