Fig 3. Expression of biofilm-associated genes in carbapenem-resistant A. baumannii ST191 isolates in planktonic conditions.
Bacteria were cultured in polystyrene tubes for 24 h at 30°C with shaking. All bacterial cells were collected for RNA extraction. Transcriptional levels of the abaI, bap, pgaA, pgaC, csuC, csuD, and csuE genes in carbapenem-resistant A. baumannii isolates were determined using RT-qPCR. The dots represent the mean of expression levels of genes in each isolate relative to expression of genes in A. baumannii ATCC 19606T and the values indicates the mean of gene expression for the entire group. Data were obtained from two independent experiments, each performed in triplicate.