Fig 1. Survival and growth of juvenile Fasciola hepatica in vitro over 29 weeks following excystment.

Juvenile fluke were maintained in: RPMI; 10% Foetal Bovine Serum (FBS) in RPMI; 5%, 10%, 20% or 50% Chicken Serum (CS) in RPMI; and, 100% CS. A—Percentage survival of juvenile F. hepatica over 29 weeks (mean±SEM). Statistical analyses were performed using One Way ANOVA with Dunnett’s post hoc test. ****, P<0.0001. B—Surface area of juvenile F. hepatica in mm² (mean±SEM). Statistical analyses were performed using Kruskal-Wallis with Dunn’s post hoc test and One Way ANOVA with Dunnett’s post hoc test. **, P<0.01.