. 2016 Sep 13;10(9):e0004994. doi: 10.1371/journal.pntd.0004994

Table 1. Excretory/secretory proteins of F. hepatica from fluke grown in 20% or 50% CS in RPMI or 100% CS for 29 weeks in vitro.

Annotation	NCBI/ Genbank Accession no.	Annotation¹^,²	% AA identity	20%	50%	100%	NEJ	Immature	Adult
Annotation	NCBI/ Genbank Accession no.	Annotation¹^,²	% AA identity	CS	CS	CS	NEJ	Immature	Adult
CL1A	Q24940.1	Q24940 ¹	97	+	+	+	-^*	+^*	+^*
CL2	ABQ95351.1	A5Z1V3¹	99	+	+	+	-^*	+^*	+^*
CL3	ABW24657.1	A8W638¹	100	+	+	+	+^*^#	+^*	-^*^##
CL3	ABW24657.1	DQ534446 ²	100	+	+	+	+^*^#	+^*	-^*^##
CL5	AAF76330.1	Q9NB30 ¹	95	+	+	-	-^*	+^*	+^*
Cathepsin B2		Q817B2 ¹	99	-	+	-	-^*	+^*	-^*
CB3	CAO98753.1	DQ534445 ²	97	-	+	+	+^#	n/a	-^##
Putative cathepsin B7		Fhep44e10.q1k¹	98	-	+	+	-^*	-^*	+^**
Legumain-like precursor 1	CAC85636	Q711M2 ¹	95	-	+	+	+^*	+^*	-^*
Legumain 1	ABQ02437.1	A6Y9U8¹	99	-	+	+	-^*	+^*	+^**
Putative legumain 2		Fhep29h09.q1k¹	100	+	+	+	+^*	-^*	-^*
Putative legumain 5		Fhep21f02.q1k¹	97	-	+	-	-^*	-^*	+^**
Putative prolylcarboxy- peptidase (s28)		Fhep30b01.q1k¹	99	-	+	+	-^*	+^*	+^*
Thioredoxin H-type 1	Q9U1G7	Q9U1G7 ¹	100	+	-	-	-^*	-^*	+^*
GST-Sigma 1	2WB9	Q06A71 ¹	97	+	+	-	-^*	+^*	-^*

Proteins observed are annotated based on the percentage (%) of amino acid (AA) identity to previously described sequences (NCBI/ GenBank, UniProtKB).

¹Robinson et al. [27] annotation;

*Robinson et al. [27] (MS/MS data);

**Robinson et al. [27] (transcript data);

²Cancela et al. [25] annotation;

^#Cancela et al. [25] (MS/MS data);

^## Cancela et al. [25] (transcript data);

n/a: not available