Table 2. PISA interface analysis of DENV C-terminal NLS Impα bond forming residues.

	C-terminal NLS	Distance [Å]	Impα	Bond*
DENV2	TYR 882 [OH]	3.55	GLU 354 [OE2]	H
	PRO 884 [O]	2.85	ARG 238 [NH1]	H
	SER 885 [O]	3.18	TRP 231 [NE1]	H
	MET 886 [O]	2.86	ASN 235 [ND2]	H
	ARG 888 [O]	2.87	TRP 184 [NE1]	H
	ARG 888 [O]	3.04	ASN 188 [ND2]	H
	ARG 890 [O]	3.04	TRP 142 [NE1]	H
	ARG 890 [O]	2.99	ASN 146 [ND2]	H
	LYS 887 [NZ]	3.10	GLY 150 [O]	H
	LYS 887 [NZ]	2.68	THR 155 [OG1]	H
	LYS 887 [NZ]	2.80	ASP 192 [OD1]	H
	ARG 888 [N]	2.73	ASN 188 [OD1]	H
	ARG 888 [NH1]	2.71	ASN 228 [OD1]	H
	ARG 888 [NH2]	2.88	ASN 228 [OD1]	H
	ARG 890 [N]	2.93	ASN 146 [OD1]	H
	ARG 890 [NH1]	2.63	GLN 181 [OE1]	H
	GLU 892 [N]	3.08	SER 105 [O]	H
	LYS 887 [NZ]	2.80	ASP 192 [OD1]	SB
DENV3	TYR 882 [OH]	3.84	ARG 315 [NH2]	H
	PRO 884 [O]	2.96	ARG 238 [NH1]	H
	SER 885 [O]	3.00	TRP 231 [NE1]	H
	MET 886 [O]	2.86	ASN 235 [ND2]	H
	ARG 888 [O]	2.95	ASN 188 [ND2]	H
	ARG 888 [O]	2.94	TRP 184 [NE1]	H
	ARG 890 [O]	3.03	TRP 142 [NE1]	H
	ARG 890 [O]	2.84	ASN 146 [ND2]	H
	TYR 882 [OH]	3.44	GLU 354 [OE2]	H
	LYS 887 [NZ]	2.79	ASP 192 [OD1]	H
	LYS 887 [NZ]	2.82	THR 155 [OG1]	H
	LYS 887 [NZ]	3.07	GLY 150 [O]	H
	ARG 888 [N]	2.72	ASN 188 [OD1]	H
	ARG 888 [NH1]	2.65	ASN 228 [OD1]	H
	ARG 888 [NH2]	2.80	ASN 228 [OD1]	H
	ARG 890 [N]	2.78	ASN 146 [OD1]	H
	ARG 890 [NH1]	2.63	GLN 181 [OE1]	H
	GLU 892 [N]	2.67	SER 105 [O]	H
	LYS 887 [NZ]	2.79	ASP 192 [OD1]	SB

* H = hydrogen bond, SB = salt bridge