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. 2016 Sep 13;11(9):e0162906. doi: 10.1371/journal.pone.0162906

Fig 2. Pull-down experiments demonstrate that the BESS domain is sufficient for BEAF-BEAF interactions.

Fig 2

A. Schematic of 32B, indicating the parts derived from 32B that had an N-terminal FLAG tag. Numbers indicate the first and last amino acid present in the truncated proteins. B. Western blot analysis of pull-down experiments. Proteins were co-expressed in E. coli and immunoprecipitated with antibodies directed against the FLAG epitope. Left panels show input proteins and right panels show immunoprecipitated proteins detected with anti-BEAF (top panels) or anti-FLAG (bottom panels) antibodies. Pull-down of 32B is only observed if the FLAG-tagged protein has a BESS domain (upper right panel).