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. 2016 Sep 13;11(9):e0162698. doi: 10.1371/journal.pone.0162698

Fig 3. SPARC colocalizes with TGFBI in mesothelial-derived ECM.

Fig 3

(a) Western blot analysis was performed on RIPA soluble lysates harvested from Met5a cells transfected with either YFP alone or SPARC-YFP. Immunoblotting with anti-GFP antibody recognizes YFP fusion constructs. (b) Extracellular matrix preparation was carried out from Met5a cells transfected with SPARC-YFP. Confocal microscopy was performed following immunostaining for YFP, TGFBI, fibronectin, and SPARC as indicated. Merged images are indicated. Scale bar 40 μm.