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. 2016 Sep 13;11(9):e0162698. doi: 10.1371/journal.pone.0162698

Fig 4. SPARC directly interacts with TGFBI via its carboxy-terminus.

Fig 4

(a) In vitro GST-binding assays. Coomassie stained gel of purified GST and GST-SPARC (aa 18–303) expressed in bacteria. Western blot analysis was performed following GST pull-down from SKOV3 lysates, probed with antibodies specific to the indicated proteins. (b) Coomassie stained gel of bacterially expressed and purified GST, GST-SPARC (aa 18–303), GST-SPARC Nterm (aa 18–134), and GST-SPARC Cterm (aa 154–303) fusion proteins. Western blot analysis was performed following GST pull-down assay from SKOV3 lysates, probed with antibodies specific to the indicated proteins. (c) In vitro binding of purified GST-SPARC to bacterially expressed and purified recombinant TGFBI. GST, GST-SPARC (aa 18–303), or GST-SPARC Nterm (aa 18–134) fusion proteins were incubated with rTGFBI or fibronectin, followed by pull-down with Glutathione sepharose 4B beads. Subsequently, Western blot analysis was performed with antibodies specific to the indicated proteins. Coomassie stained gel represents experimental input.