FIG 4.

Heterologous membrane binding sequences can replace HIV-1 MA without affecting VCC localization. (A) Schematic illustrations of WT, PH/ΔMA, Kmyr/ΔMA, and Fyn(10)/ΔMA Gag-YFP. (B) MDMs were infected with pseudotyped HIV-1 encoding WT Gag-YFP or Gag-YFP derivatives in which MA was replaced by a heterologous membrane binding sequence. At 48 h postinfection, cells were stained with ConA labeled with Alexa Fluor 594, fixed, immunostained with a mouse monoclonal anti-CD81 antibody and anti-mouse IgG conjugated with Alexa Fluor 647, and analyzed using a confocal microscope. (C) Pearson's correlation coefficients for colocalization of Gag-YFP with CD81 are shown as means and SEM. Twenty to 30 cells were analyzed per condition. n.s., not significant.