FIG 6.

Low frequencies of autoreactivity and polyreactivity of MPER-specific Abs elicited by immunization with various MPER/liposome vaccines. (A) Forty-four different MPER-specific rMAbs from mice immunized with liposome vaccines containing Npalm-MPER, the Npalm-W680A mutant, or Cpalm-MPER were tested for autoreactivity and polyreactivity by cardiolipin and dsDNA ELISAs and were compared with anti-MPER BNAbs (red, 2F5; purple, 4E10; and green, 10E8). Data shown are representative of three independent experiments. (B) Lack of correlation between reactivities and CDRH3 lengths among the 44 different Abs tested in panel A. The y axis represents the absorbance at 490 nm of bound antibodies from panel A at 5 μg/ml, plotted against CDRH3 length on the x axis.