FIG 9.

Reverse correlation between MPER binding and polyreactivity determined by chain swap and/or CDRH3 mutations. (A) IgH and IgLk V(D)J gene usage of related Abs 196 and 219. 219M is a 219 variant in which CDRH3 of clone 219 was replaced by CDRH3 of clone 196. (B) Relative binding reactivities to MPER/liposomes as assessed by SPR analysis of chimeric rMAbs from clones 196 and 219 compared to that of naturally paired clone 196 (left panel). Also shown are 219M binding reactivities for MPER/liposomes (middle) and liposomes only (right) in comparison to those of WT clones 196 and 219. Thirty microliters of each antibody at 100 μg/ml was injected over the MPER/liposome or liposome surface of an L1 chip at a flow rate of 10 μl/min for 3 min. (C) rMAbs were tested for polyreactivity by cardiolipin and dsDNA ELISAs.