FIG 1.

Interaction network of retrograde transport factors overlaid with siRNA hits. The 158 genes annotated in the endosome-to-Golgi retrograde traffic category (GO:0042147) plus human Rab membrane GTPase genes and additional literature-curated genes designated to function in the retrograde pathway were compiled. Using the Ingenuity Pathway Analysis protein-protein interaction database, we made an interaction map from this list and overlaid the median Z-score of 5 siRNAs for each gene from our siRNA screen results (28). Decrease of virus spread is depicted in shades of red from dark to light, while increase is shown in green colors. Uncolored circles indicate spread that was unchanged or not evaluated. Continuous arrows connect proteins that have been shown to interact, dashed arrows connect proteins for which interactions have not been shown to be direct, and circular lines indicate self-associations. The symbols are defined at the bottom of the figure.