FIG 3.
IFN-γ secretion in HIV-1 Gag- and Pol-specific CD8+ T cells cocultured with HIV-1-infected cells expressing RhT5 and TCyp. RhT5- and TCyp-expressing cells were infected with HIV-1GFP at an MOI of 0.5 and cocultured with HLA class I-matched HIV-1 Gag- or Pol-specific CD8+ T cells at various effector/target (E/T) ratios (1:16, 1:8, 1:4, and 1:2) and incubated overnight. (A) IFN-γ spots per well in Gag-specific CD8+ T cells. (B) IFN-γ spots per well in Pol-specific CD8+ T cells. Data are from two independent experiments performed in triplicate for panel A and from three independent experiments performed in triplicate for panel B. The P values were calculated using a Wilcoxon signed-rank test. Only significant values are shown in the figure (***, P < 0.0001; **, P < 0.001; *, P < 0.01).