Effects of mutation(s) in UL51 and/or UL14 on progeny virus yields. (A and B) Vero cells were infected with HSV-1(F), YK5015 (ΔUL14), YK5016 (ΔUL14-repair), YK5011 (ΔUL51), YK5012 (ΔUL51-repair), YK5013 (UL51LIY/AAA), YK5014 (UL51LIY/AAA-repair), YK5017 (ΔUL51/ΔUL14), or YK5018 (ΔUL51/ΔUL14-repair) at an MOI of 5 (A) or 0.01 (B). Total virus from cell culture supernatants and infected cells was harvested at 18 h (A) or 48 h (B) postinfection and assayed on Vero cells. Each data point is the mean ± standard error from the results for triplicate samples. Differences in viral yields between HSV-1(F) and each of the mutant viruses were statistically significant by ANOVA and Dunnett's test (P < 0.05). Differences in viral yields between HSV-1(F) and each of the repaired viruses were not statistically significant by ANOVA and Dunnett's test. Data are representative of three independent experiments.