TABLE 2.
Effects of the mutations in UL51 and/or UL14 on the distribution of viral particles in HSV-1-infected Vero cells
| Virus | % of virus particles in morphogenetic stage (no. of particles in stage) |
Total no. counted (particles/cells) | |||||
|---|---|---|---|---|---|---|---|
| Capsids in nucleus | Enveloped virions in perinuclear space | Unenveloped capsids in cytoplasm | Partially enveloped capsids in cytoplasm | Enveloped virions in cytoplasm | Extracellular enveloped virions | ||
| HSV-1(F) | 44.0 (521) | 6.8 (81) | 5.2 (62) | 4.1 (49) | 15.8 (187) | 24.1 (285) | 1,185/8 |
| ΔUL14 | 38.4 (308) | 4.6 (37) | 29.8 (239) | 11.8 (95) | 8.3 (67) | 7.1 (57) | 803/8 |
| ΔUL14-repair | 39.8 (449) | 4.5 (51) | 11.0 (124) | 3.5 (39) | 21.3 (240) | 19.9 (224) | 1,127/8 |
| ΔUL51 | 38.0 (353) | 7.3 (68) | 30.0 (278) | 12.1 (112) | 4.2 (39) | 8.4 (78) | 928/8 |
| ΔUL51-repair | 42.9 (438) | 7.1 (73) | 8.9 (91) | 1.7 (17) | 17.1 (175) | 22.2 (227) | 1,021/8 |
| UL51LIY/AAA | 45.8 (352) | 3.0 (23) | 26.3 (202) | 12.0 (92) | 5.5 (42) | 7.5 (58) | 769/8 |
| UL51LIY/AAA-repair | 36.9 (404) | 9.1 (100) | 10.0 (109) | 3.7 (41) | 18.4 (202) | 21.8 (239) | 1,095/8 |
| ΔUL51/ΔUL14 | 43.2 (328) | 3.6 (27) | 32.8 (249) | 11.3 (86) | 3.6 (27) | 5.5 (42) | 759/8 |
| ΔUL51/ΔUL14-repair | 39.8 (382) | 5.3 (51) | 8.3 (80) | 1.7 (16) | 18.2 (175) | 26.6 (255) | 959/8 |
| ΔUL13 | 48.1 (494) | 5.4 (55) | 5.2 (53) | 3.1 (32) | 17.7 (182) | 20.5 (210) | 1,026/8 |