FIG 7.

Mutation F291I in E1 confers resistance to B5 treatment. Huh-7 cells were pretreated for 1 h with B5 before infection with JFH1 or mutant viruses (MOI of 1), and infected cells were in contact with the drug until the end of the experiment. At 30 h postinfection, infected cells were quantified. Results are expressed as percentage of infection in the presence of the solvent. Error bars indicate standard errors of the means from at least three independent experiments. Mutant FYM contains E1 mutations F291I, Y297H, and M356V. Mutant FY contains E1 mutations F291I and Y297H. Mutants F and Y contain mutations F291I and Y297H, respectively.