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Time course of macro domain and macro-Hel activity on mono-ADP-ribosylated PARP15 (A) and poly(ADP)-ribosylated PARP5a (B) catalytic domains. The auto-ADP-ribosylated PARP5a or -15 catalytic domain was incubated with 100 nM macro domain or macro-Hel at 30°C for 1 h or 2 h. PSL-BG, photo-stimulated luminescence counts minus background counts.
