. 2016 Sep 12;90(19):8478–8486. doi: 10.1128/JVI.00705-16

TABLE 3.

List of macro-Hel mutants in the helicase domain

Hel protein	Predicted function^a	Location of mutation^a	Relative activity (%)^b
Hel protein	Predicted function^a	Location of mutation^a	ATPase	ssRNA binding
Wild type			100	100
K215A	NTP binding	Walker A motif	5.6 ± 4.5	54.9 ± 1.6
Q291A	NTP binding	Motif III	0	115.7 ± 7.11
R321A	NTP binding	Motif IIIa	0.4 ± 1.4	54.2 ± 3.4
Q378A	NTP binding	Motif Va	39.0 ± 4.2	120.7 ± 2.7
R401A	ssRNA binding	Motif VI	72.6 ± 8.6	90.7 ± 2.6
D431A	ND^c		79.0 ± 2.5	59 ± 3.6

The function and location of mutated residues were predicted based on the sequence alignment with the SF1 helicase from tomato mosaic virus (50).

The ATPase and ssRNA binding activities were determined relative to that of the wild type, which was set as 100%. Values are means ± standard deviations (n = 3).

ND, not determined.