FIG 3.

Ability of W69 variants to interact with ligands that prefer the CD4-bound conformation. Comparable amounts of radiolabeled monomeric gp120 from WT and W69 variants were incubated with 5 μg/ml of 17b (A), 48d (B), A32 (C), N5-i5 (D), and N60-i3 (E) for 1 h at 37°C. The precipitates were washed, run on SDS-polyacrylamide gels, and analyzed by densitometry. All values shown here were evaluated by the ligand binding capacity of WT gp120 (set as 1). Data represent the averages ± SD from at least three independent experiments. Statistical significance was tested using an unpaired two-tailed t test. Shown are P values with significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).