FIG 3.

Temporal analysis of MIE transcript abundance. (A) Primary fibroblasts were infected with HCMV (MOI of 3). The abundance of the indicated MIE transcript at each time point was measured by qRT-PCR using the absolute abundance method as described in Materials and Methods. (B) Cells were infected as described above for panel A in the presence of cycloheximide (gray bars) or absence of cycloheximide (black bars). The relative abundance of the indicated transcript was measured by qRT-PCR at 6 h after infection. Transcript abundance in untreated cells was set at one. (C) Cells were infected as in panel A in the presence (open bars) or absence (black bars) of PAA. Transcript abundance was measured by qRT-PCR using the absolute abundance method. Values that are significantly different (P value < 0.001) are indicated by a bar and asterisk. (D) Cells were infected and treated as in panel C. The reduction of UL99 mRNA expression in the presence of PAA is shown for comparison. NT, not treated.