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. 2016 Aug 30;2016:4239375. doi: 10.1155/2016/4239375

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Copyright © 2016 Yulia Sokurenko et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Electrophoretic analysis of purity, enzymatic activity, and antibody specificity of balifase. (a) SDS/PAGE of balifase samples at different stages of purification. 1: before purification in culture fluid, 2: after chromatography on DEAE-cellulose, 3: after chromatography on phosphocellulose P-11, 4: after chromatography on UNOS₆ column, 5: binase. (b) Zymography analysis of balifase sample. 1: after chromatography on phosphocellulose P-11; 2: after chromatography on UNOS₆ column; 3: binase. (c) Zymography analysis of balifase sample after concentrating, freezing, and storage. 1: balifase; 2: binase. (d) Western Blot analysis of balifase after chromatography on UNOS₆ column. 1: binase; 2: balifase.