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. 2016 Aug 30;2016:5623089. doi: 10.1155/2016/5623089

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Copyright © 2016 Axel Poulet et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Performance of AGSA software for evaluation of homopolymers. 299 indel variants were found by AGSA in homopolymer sequences. After analysis of individual flowgrams, 246 (86%) were classified as false positive variants and 43 (14%) as true variants. Sanger sequencing confirmed that the 246 AGSA-classified false positives were actually wild-type sequences. Among the 43 potentially real variants, 29 (10%) were confirmed with Sanger analysis and 14 (5%) were actually wild-type Sanger sequences.