Table 1.
Summary of culture substrates used for culturing undifferentiated hPSCs.
| Name | Commercial name (if any, only major) | Material type | References |
|---|---|---|---|
| Gelatinous protein mixture | Matrigel (Corning) | Crude extract secreted by EHS mouse sarcoma cells | [9, 28] |
| Geltrex (Thermo Fisher Scientific) | |||
| Cultrex BME (Trevigen) | |||
|
| |||
| Laminin | Extracted protein | [9] | |
|
| |||
| Laminin-511 | LN511 (Biolamina) | Recombinant protein | [29, 30] |
|
| |||
| Laminin-521 | LN521 (Biolamina) | Recombinant protein | [31, 32] |
| rhLaminin-521 (Thermo Fisher Scientific) | |||
|
| |||
| Laminin-E8 fragment | iMatrix-511 (Nippi) | Recombinant protein | [33, 34] |
|
| |||
| Vitronectin | Vitronectin XF (Stem Cell Technologies) | Extracted or recombinant protein | [35–38] |
|
| |||
| Truncated vitronectin | VTN-N (Thermo Fisher Scientific) | Recombinant protein | [39] |
|
| |||
| Fibronectin | Extracted protein | [40–42] | |
|
| |||
| Collagen type I | Extracted protein | [20, 43] | |
|
| |||
| Nanofibrous gelatin | Processed gelatin | [44] | |
|
| |||
| Customized spider silk protein | Recombinant protein, containing vitronectin motif | [45] | |
|
| |||
| Peptide-acrylate surfaces (PAS) | Synthemax (Corning) | Synthetic polymers with peptides | [46] |
|
| |||
| PMEDSAH | Synthetic polymer | [47] | |
|
| |||
| Synthetic substrates displaying heparin-binding peptides | Synthetic polymers with peptides | [48] | |
|
| |||
| Polyvinylalcohol-co-itaconic acid hydrogels grafted with aoligopeptide derived from vitronectin (KGGPQVTRGDVFTMP) | Synthetic polymers with peptides | [49] | |
|
| |||
| UV/ozone radiation | Modification of typical cell culture plastics | [50] | |
|
| |||
| Hydrogel interfaces of aminopropylmethacrylamide (APMAAm) | Synthetic polymers | [51] | |
|
| |||
| Human E-cadherin-Fc chimeric protein | Recombinant protein | [31, 52] | |