Figure 4.

Relative mRNA expression of HIF-1α, CAT, GPx1, and SOD1 in colon, MLN, and spleen (a, b) and their correlation to the HIF-1α gene expression in colonic tissue (c). Relative mRNA expression was measured by real-time PCR; all measured genes were normalized to the HPRT1 gene expression. BALB/c mice at the age of 10–12 weeks were randomly assigned into 4 groups (n = min. 5/group/experiments): CTRL: control mice, CTRL + HBO₂: control mice undergoing HBO₂ (60 min/2.4 ATM, 2x/day, days 1–8), DSS: mice receiving dextran sodium sulphate (DSS, 5% w/v, days 1–7), and DSS + HBO₂: DSS treated mice undergoing HBO₂. Data are presented as mean ± s.e.m. of two independent experiments, each with min. 5 mice/group. ^∗Statistically different from CTRL, P < 0.05; ^†statistically different from CTRL + HBO₂, P < 0.05.