(
A) Light membranes (M) were incubated with an energy regenerating mix and either buffer or 5 µM cytLnp for 15 min. The membranes were stained with octadecyl rhodamine. Scale bar = 5 µm. (
B) Buffer or 5 µM of cytLnp was added to a preformed network formed from cytosol (
C) and membranes (M). Scale bar = 5 µm. (
C) Buffer or 200 nM of affinity-purified Lnp antibody was added to a preformed network formed from membranes only. Scale bar = 5 µm. (
D) Interaction of endogenous
Xenopus egg Lnp with various Lnp fragments. A detergent extract of light membranes was incubated with empty resin or hemagglutinin (HA)-tagged Lnp fragments (see
Figure 11A). After pull-down with HA antibodies, the bound fractions were analyzed by immunoblotting with antibodies to
Xenopus Lnp. (
E) Interaction of purified
Xenopus cytLnp with various cytLnp fragments. HA-tagged Lnp fragments (see
Figure 11A) was incubated with cytLnp, and subsequently HA antibodies. Bound fractions were analyzed by SDS-PAGE and stained with Coomassie blue. (
F) Purified cytLnp or fragments of it (see
Figure 11A) were analyzed by gel filtration on a Superdex 200 column.