Figure 6. Effect of GTP analogs on ER network formation and maintenance.
GDP-BeF3- was added at 0.67 mM or 2 mM either before (upper panels) or after (lower panels) formation of an ER network from Xenopus egg cytosol (C), light membranes (M), and an energy regenerating system. The membranes were stained with octadecyl rhodamine. Scale bar = 10 µm.
Figure 6—figure supplement 1. ER network maintenance is less sensitive to ATL inhibition than ER network formation.
GTPγS was added at 1 mM or 2 mM either before (upper panels) or after (lower panels) formation of an ER network from Xenopus egg cytosol (C), light membranes (M), and an energy regenerating system. The membranes were stained with octadecyl rhodamine. Scale bar = 10 µm.