Video 2. ER in U2OS cells expressing dominant-negative ATL-1.
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DOI: 10.7554/eLife.18605.006
U2OS cell stably expressing GFP-ATL-1 K80A (GTPase-defective mutant) with many unbranched tubules and a fragmented peripheral ER. Images were acquired with a spinning disk confocal microscope at 0.05 sec intervals for 5 sec. The video is displayed at the same rate it was acquired (20 frames per sec). This cell is also depicted in Figure 1— figure supplement 1A, left panel. Image scale: 87 × 66 µm.