Video 6. ER morphology in U2OS cells after overexpression of Rtn4a.
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DOI: 10.7554/eLife.18605.020
U2OS cells expressing GFP-calreticulin under the endogenous promoter as well as stably expressing mCherry- Rtn4a. Left panel: calreticulin signal, right panel: mCherry-Rtn4a signal. Images were acquired with a spinning disk confocal microscope at 0.4 sec intervals for 4 sec. The video is displayed at the same rate it was acquired (2.5 frames per sec). Image scale: 87 × 66 µm for each panel.