. Author manuscript; available in PMC: 2017 Oct 1.

Published in final edited form as: Hum Mutat. 2016 Aug 21;37(10):1051–1059. doi: 10.1002/humu.23043

Table 1. Open intervals and burst durations of wild-type and mutant AChRs on HEK cells.

	Open Intervals			Bursts
	τ₁, ms	τ₂, ms	τ₃, ms	τ₁, ms	τ₂, ms	τ₃, ms
	(a₁)	(a₂)	(a₃)	(a₁)	(a₂)	(a₃)
Wild type	0.037 ± 0.033^a	0.31 ± 0.050	1.35 ± 0.051	0.036 ± 0.0017^b	0.47 ± 0.059	3.31 ± 0.12
	(0.17 ± 0.022)	(0.27 ± 0.038)	(0.67 ± 0.042)	(0.24 ± 0.021)	(0.21 ± 0.027)	(0.58 ± 0.038)
βV266A	0.068 ± 0.0072	0.85 ± 0.087	2.27 ± 0.10	0.073 ± 0.0068	0.88 ± 0.21	13.35 ± 0.87
(p.Val289Ala)	(0.20 ± 0.0050)	(0.55 ± 0.031)	(0.25 ± 0.030)	(0.41 ± 0.041)	(0.20 ± 0.045)	(0.39 ± 0.046)
εV265A	0.035 ± 0.0025	0.31 ± 0.030	2.61 ± 0.010	0.048 ± 0.0070	0.44 ± 0.020	14.33 ± 0.31
(p.Val285Ala)	(0.18 ± 0.0080)	(0.18 ± 0.027)	(0.65 ± 0.019)	(0.43 ± 0.020)	(0.29 ± 0.033)	(0.28 ± 0.014)
εV265G	0.080 ± 0.011	1.34 ± 0.33	5.30 ± 0.38	0.082 ± 0.0026	1.60 ± 0.39	24.46 ± 3.07
(p.Val285Gly)	(0.090 ± 0.013)	(0.30 ± 0.026)	(0.61 ± 0.022)	(0.13 ± 0.013)	(0.24 ± 0.063)	(0.64 ± 0.050)
εV265L	0.032 ± 0.0079	0.52 ± 0.039	3.80 ± 0.66	0.035 ± 0.0090	0.39 ± 0.025	10.82 ± 1.10
(p.Val285Leu)	(0.36 ± 0.061)	(0.21 ± 0.028)	(0.44 ± 0.071)	(0.52 ± 0.056)	(0.21 ± 0.048)	(0.28 ± 0.095)
αV255A	0.046 ± 0.0052	0.34 ± 0.022	6.31 ± 0.48	0.079 ± 0.012	0.55 ± 0.083	26.59 ± 2.70
(p.Val275Ala)	(0.23± 0.020)	(0.09 ± 0.0040)	(0.67 ± 0.028)	(0.32 ± 0.055)	(0.12 ± 0.017)	(0.56 ± 0.053)
δV269A	0.042 ± 0.0034	0.35 ± 0.073	4.72 ± 0.77	0.047± 0.0076	0.59 ± 0.090	13.90 ± 1.89
(p.Val290Ala)	(0.22 ± 0.057)	(0.11 ± 0.028)	(0.67 ± 0.079)	(0.30 ± 0.096)	(0.10 ± 0.012)	(0.60 ± 0.099)

Values indicate mean ± SE of 21 patches for wild-type and at least 3 patches for all mutants. All patches with ACh 50 nM at final bandwidth 11.7 -12 kHz. MP = -80 mV.

^{a, b}

τ₁ components of opening intervals and bursts were not detected at 12 and 3 patches, respectively, in wild-type AChR. HGVS nomenclature is shown in parentheses.